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In this context of limited response to single target agents, novel candidate molecules capable of effectively inducing tumor inhibition along with targeting multiple critical nodes of melanoma signaling assume translational significance. In this regard, we investigated the anti-cancer effects of a novel dichlorophenyl urea compound called COH-SR4 in melanoma. The SR4 treatment decreased the survival and inhibited the clonogenic potential of melanomas along with inducing apoptosis in vitro cultures. SR4 treatments lead to inhibition of GST activity along with causing G2/M phase cell cycle arrest. Oral administration of 4mg/kg SR4 leads to effective inhibition of tumor burdens in both syngeneic and nude mouse models of melanoma. The SR4 treatment was well tolerated and no overt toxicity was observed. The histopathological examination of resected tumor sections revealed decreased blood vessels, decrease in the levels of angiogenesis marker, CD31, and proliferation marker, Ki67, along with an increase in pAMPK levels. Western blot analyses of resected tumor lysates revealed increased PARP cleavage, Bim, pAMPK along with decreased pAkt, vimentin, fibronectin, CDK4 and cyclin B1. Thus, SR4 represents a novel candidate for the further development of mono and combinatorial therapies to effectively target aggressive and therapeutically refractory melanomas.

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Melanoma arises from the malignant transformation of the pigmented cells of the skin called melanocytes. Click here for webinar recordings.The incidence of cutaneous melanoma has been rising in the past decade.

Register for the next Secure Webinar by visiting the link in the table below. These sessions will run throughout the year, on the third week of each month. Each session will feature an overview of our cyber solutions, customer success stories, and interactive demonstrations.

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The AutomaTech-Secure Webinars are sessions designed to help you learn how to implement practical and realistic security measures to protect your critical industrial infrastructure. Register for the next webinar by visiting the link in the table below. Featuring real-world use cases and interactive demonstrations. Click here for session recordings.ĪutomaTech-Education Webinars are 60-minute sessions designed to help you understand how operational improvements can be gained by leveraging the latest industrial tech. Register for upcoming sessions by visiting the link in the table below. The AutomaTech-Connect Tech Showcase Demo series are led by our Solution Architect team, and will feature hour-long live and interactive web demonstrations of the latest AutomaTech technology.

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